After transcardial perfusion with cold 100 mL PBS in rats and 40 mL in mouse, lumbar (L) 4 and 5 DRG lumbar spinal cord, and sciatic nerve segment proximal to the sciatic bifurcation were dissected, and fixed in Richard-Allan Scientific™ Buffered Zinc Formalin (ThermoFisher, Rockford, IL) overnight, followed by processing for paraffin embedment. The previously described histological protocol was adopted.26 (link)
For western blot experiments, L4/L5 DRG were removed, snap frozen in liquid nitrogen, and stored at −80°C for extraction of protein.
For western blot experiments, L4/L5 DRG were removed, snap frozen in liquid nitrogen, and stored at −80°C for extraction of protein.
