Cell Migration Assay for Wound Healing

The assay was performed as described by Đorđevski et al. [49 (link)] with some modifications. HaCaT cells were grown until reaching 85% confluence. The cell monolayer was scratched with a 200 μL sterile tip. Floating cells were washed, and cells were incubated in reduced DMEM supplemented with 1% FBS, 2 mM L-glutamine, and 1% antibiotic-antimycotic, containing 400 µg/mL of preparations. Cell migration was monitored using Nikon Eclipse TS2 (Amsterdam, the Netherlands) 48 h after wound preparation and treatment. The untreated control was used to measure wound closure under these conditions and without the addition of preparations. Results were presented as the percentage of wound closure during exposure to the tested extracts.