Influenza Virus Propagation in Eggs

Sf9 insect cells were maintained in SF900II (Life Technologies, San Diego, CA, USA) at 27 °C in cell culture. Influenza virus A/Aichi/68 H3N2 and A/Anhui/2013 H7N9 provided by Dr. Zhu Hongwei (Ludong University) were propagated in the allantoic cavities of 10-day-old embryonated hen’s eggs at 37 °C for 2 d. Allantoic fluid was harvested and centrifuged at 2000 rpm for 10 min. The viruses were titrated by infection of mice with serial dilutions, and LD₅₀ (50% lethal dose) was calculated using the method of Reed and Muench.

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Shi L., Long Y., Zhu Y., Dong J., Chen Y., Feng H, & Sun X. (2023). VLPs containing stalk domain and ectodomain of matrix protein 2 of influenza induce protection in mice. Virology Journal, 20, 38.

Publication 2023

SF900II

Allantoic Cavities Cell culture Dilutions Eggs H7n9 Infection Influenza virus a Insect Mice Sf9 cells Viruses

Corresponding Organization : Huzhou University

Other organizations : Jiaxing University

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Variable analysis

independent variables

Influenza virus A/Aichi/68 H3N2
Influenza virus A/Anhui/2013 H7N9

dependent variables

Lethal dose (LD50)

control variables

Sf9 insect cells maintained in SF900II cell culture at 27 °C
Embryonated hen's eggs at 37 °C for 2 days

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