For the L‐Dopa and Carbidopa studies, NPCs were seeded on top of mouse PA6 cells and maintained in N2B27 medium, as described above. DAn were treated after 20 days in culture with L‐Dopa (50 μM; 3,4‐dihydroxy‐L‐phenylalanine, Sigma) and Carbidopa (12.5 μM; Sigma) in a ratio of 4:1 (Burbulla et al, 2017 (link)) for 10 days. Each compound was added three times a week at each change of the medium. For the early L‐Dopa studies, treatment was started on day 6 during the neural induction of EBs and maintained until the end of differentiation on day 30, for a total of 24 days.
Tristán‐Noguero A., Fernández‐Carasa I., Calatayud C., Bermejo‐Casadesús C., Pons‐Espinal M., Colini Baldeschi A., Campa L., Artigas F., Bortolozzi A., Domingo‐Jiménez R., Ibáñez S., Pineda M., Artuch R., Raya Á., García‐Cazorla À, & Consiglio A. (2023). iPSC‐based modeling of THD recapitulates disease phenotypes and reveals neuronal malformation. EMBO Molecular Medicine, 15(3), e15847.
Other organizations :
Hospital Sant Joan de Déu Barcelona, Institut d'Investigació Biomédica de Bellvitge, Universitat de Barcelona, Bellvitge University Hospital, Center of Regenerative Medicine in Barcelona, Duran i Reynals Hospital, Institut d'Investigacions Biomèdiques de Barcelona, Consejo Superior de Investigaciones Científicas, Consorci Institut D'Investigacions Biomediques August Pi I Sunyer, Centro de Investigación Biomédica en Red de Salud Mental, Instituto de Salud Carlos III, Instituto Murciano de Investigación Biosanitaria, Centro de Investigación Biomédica en Red, Centre for Biomedical Network Research on Rare Diseases, Hospital Universitario Virgen de la Arrixaca, Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine, Institució Catalana de Recerca i Estudis Avançats, University of Brescia
L-Dopa and Carbidopa added three times a week at each medium change
controls
Early L-Dopa studies: Treatment started on day 6 during neural induction of EBs and maintained until day 30, for a total of 24 days
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