After incubation in a 96-well plate (8.0 × 103 cells/well) for 24 h, HepG2 cells were pre-treated with PRA extracts (37.5, 75, and 150 μg/ml) for 24 h, followed by incubation with H2O2 (600 μM) for 6 h. Cells only treated with H2O2 were used as model group (oxidative stress). The CCK-8 assay kit was used to determine the cytoprotective effects of PRA extracts against H2O2-induced oxidative damage.
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