In sections subjected to in situ hybridization using each V1R probe, labeled cells in the lamellae and recesses were counted, and their areas were measured using ImageJ software (https://imagej.nih.gov/ij/) as described previously [28 (link)]. The number of labeled cells in the lamellae or recesses was divided by the respective area to calculate the density of labeled cells for each probe (number of labeled cells per 1 mm2).
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