Details for reagents and antibodies described in the Methods can be found in Supplemental Table 2 .
Adult C57BL/6N mice were purchased from Shanghai SLAC Laboratory. C57BL/6N Gsdmd−/− mice were purchased from GemPharmatech. All animals were housed in a pathogen-free environment in the Tongji University animal facilities. Knockout of the Gsdmd gene was validated by genotyping and immunoblotting. All animal surgeries were performed in 10- to 14-week-old male mice.
To deplete neutrophils in mice, isotype IgG (Cell Signaling Technology), anti-Ly6G antibody (BioLegend), or anti-Ly6G/Ly6C antibody (BioLegend) was injected intraperitoneally at a dose of 200 μg per mouse, as described previously (30 (link), 31 (link)). The relevant mice were subjected to myocardial infarction surgery 24 hours later. For NSA administration, 20 mg/kg mouse weight of NSA (MedChemExpress) was injected intraperitoneally 30 minutes before AMI surgery; a second injection was administered 8 hours after the surgery. Mice in the corresponding control groups were injected with the same amount of solvent (DMSO) without NSA.
Adult C57BL/6N mice were purchased from Shanghai SLAC Laboratory. C57BL/6N Gsdmd−/− mice were purchased from GemPharmatech. All animals were housed in a pathogen-free environment in the Tongji University animal facilities. Knockout of the Gsdmd gene was validated by genotyping and immunoblotting. All animal surgeries were performed in 10- to 14-week-old male mice.
To deplete neutrophils in mice, isotype IgG (Cell Signaling Technology), anti-Ly6G antibody (BioLegend), or anti-Ly6G/Ly6C antibody (BioLegend) was injected intraperitoneally at a dose of 200 μg per mouse, as described previously (30 (link), 31 (link)). The relevant mice were subjected to myocardial infarction surgery 24 hours later. For NSA administration, 20 mg/kg mouse weight of NSA (MedChemExpress) was injected intraperitoneally 30 minutes before AMI surgery; a second injection was administered 8 hours after the surgery. Mice in the corresponding control groups were injected with the same amount of solvent (DMSO) without NSA.
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