Serological Assays for Arboviral Infections

Acute- and convalescent-phase serum samples were tested by IgG ELISA with ZIKV antigen as described for detection of IgG to arboviruses (12 (link)). Samples were also tested by IgM ELISA as described with the following viral antigens: ZIKV, DENV 1–4 mixture, yellow fever virus (YFV), Japanese encephalitis virus, and Murray Valley encephalitis virus (13 (link)). Testing for IgM to West Nile virus (WNV) and St. Louis encephalitis virus was performed by using a microsphere immunoassay (14 (link)). Ratios of patient optical density values to negative control values (P/Ns) were calculated for IgG and IgM ELISAs. Values >3 were considered positive, and values 2–3 were considered equivocal. Neutralizing antibody titers were determined by using a PRNT with a 90% cut-off value (15 (link)).

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Lanciotti R.S., Kosoy O.L., Laven J.J., Velez J.O., Lambert A.J., Johnson A.J., Stanfield S.M, & Duffy M.R. (2008). Genetic and Serologic Properties of Zika Virus Associated with an Epidemic, Yap State, Micronesia, 2007. Emerging Infectious Diseases, 14(8), 1232-1239.

Publication 2008

Antigen Arboviruses Elisas Encephalitis virus Immunoassay Japanese encephalitis virus Microsphere Murray valley encephalitis virus Neutralizing antibody Patient Ratios Serum Viral antigens West nile virus Yellow fever virus Zikv

Corresponding Organization :

Other organizations : Centers for Disease Control and Prevention

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Variable analysis

independent variables

Acute- and convalescent-phase serum samples

dependent variables

IgG ELISA with ZIKV antigen
IgM ELISA with ZIKV, DENV 1–4 mixture, yellow fever virus (YFV), Japanese encephalitis virus, and Murray Valley encephalitis virus antigens
IgM to West Nile virus (WNV) and St. Louis encephalitis virus tested by using a microsphere immunoassay
Neutralizing antibody titers determined by using a PRNT with a 90% cut-off value

control variables

Negative control values for IgG and IgM ELISAs

controls

Positive controls not explicitly mentioned
Negative controls: Negative control values for IgG and IgM ELISAs

Annotations

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