Colloidal Gold Nanoparticle Production

The SP2/0 cell line was stored and cultured by the plant quarantine and bacteriology laboratory of Nanjing Agricultural University. Colloidal gold particles (40 nm in diameter) were prepared using the trisodium citrate reduction method, as described by Contreras-Trigo et al. [21 (link)]. Bovine serum albumin (BSA), Tween-20, dimethyl sulfoxide (DMSO), 3,3′,5,5′-tetramethylbenzidine, Freund’s complete/incomplete adjuvants and polyethylene glycol (PEG1500) were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Hypoxanthine aminopterin thymidine (HAT), hypoxanthine thymidine (HT) and Dulbecco’s Modified Eagle Medium (DMEM) were provided by Gibco (Grand Island, NY, USA). Horseradish peroxidase-labeled goat anti-mouse IgG conjugate (HRP-GaMIgG) was acquired from Zhuyan Biological Technology Co., Ltd. (Nanjing, China). Fetal bovine serum (FBS) was provided by Lanzhou Minhai Biological Engineering Co., Ltd. (Lanzhou, China).

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Zhao Z., Tian Y., Xu C., Xing Y., Yang L., Qian G., Hua X., Gong W., Hu B, & Wang L. (2023). A Monoclonal Antibody-Based Immunochromatographic Test Strip and Its Application in the Rapid Detection of Cucumber Green Mottle Mosaic Virus. Biosensors, 13(2), 199.

Publication 2023

Bovine serum albumin (BSA), Tween-20 Freund’s complete/incomplete adjuvants polyethylene glycol (PEG1500) Dulbecco’s Modified Eagle Medium (DMEM)

Adjuvants Aminopterin Anti igg Biological Bovine serum albumin Cell line Colloidal gold Dimethyl sulfoxide Eagle Fetal bovine serum Goat Hypoxanthine Mouse Plant Polyethylene glycol Quarantine Tetramethylbenzidine Thymidine Trisodium citrate Tween 20

Corresponding Organization : Nanjing Agricultural University

Other organizations : Institute of Plant Protection

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Variable analysis

independent variables

Colloidal gold particles (40 nm in diameter)

dependent variables

Measured outcomes

control variables

Bovine serum albumin (BSA)
Tween-20
Dimethyl sulfoxide (DMSO)
3,3′,5,5′-tetramethylbenzidine
Freund's complete/incomplete adjuvants
Polyethylene glycol (PEG1500)
Hypoxanthine aminopterin thymidine (HAT)
Hypoxanthine thymidine (HT)
Dulbecco's Modified Eagle Medium (DMEM)
Horseradish peroxidase-labeled goat anti-mouse IgG conjugate (HRP-GaMIgG)
Fetal bovine serum (FBS)

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

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