PB or BM aspirates from 80 patients were cultured under stimulated culture conditions and harvested using routine laboratory protocol. Conventional karyotype analysis was performed with G-banded metaphase cells obtained after 72 h of lipopolysaccharide stimulation of the BM aspirates. In each case, at least 20 metaphase cells were assessed, and the cytogenetic abnormalities were interpreted following the 2016 International System for Human Cytogenetic Nomenclature guidelines [64 ].
FISH analysis was performed on 105 patients following the manufacturer’s instructions. Anomalies were detected using five probes: MYB Deletion Probe for del(6q), ATM Deletion Probe for del(11q), Chromosome 12 Alpha Satellite Probe for trisomy 12, RB1 Deletion Probe for del(13q), and TP53 Deletion Probe for del(17p) (Cytocell, Banbury, UK). Slides were prepared using cells harvested for conventional cytogenetic studies and processed for FISH. A total of 400 interphase or metaphase nuclei were examined in each case.
FISH analysis was performed on 105 patients following the manufacturer’s instructions. Anomalies were detected using five probes: MYB Deletion Probe for del(6q), ATM Deletion Probe for del(11q), Chromosome 12 Alpha Satellite Probe for trisomy 12, RB1 Deletion Probe for del(13q), and TP53 Deletion Probe for del(17p) (Cytocell, Banbury, UK). Slides were prepared using cells harvested for conventional cytogenetic studies and processed for FISH. A total of 400 interphase or metaphase nuclei were examined in each case.
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