Single-nucleotide polymorphism (SNP) genotyping had been implemented in previous reports (Li F. et al., 2014 (link); Li et al., 2016 (link)). Briefly, genomic DNA of 472 rapeseed accessions was extracted from the young leaf tissue and was then hybridized to the Brassica 60K Illumina® Infinium SNP array, which contains 52,157 SNP markers, according to manufacturer’s protocol. The SNPs with minor allele frequency (MAF) < 5% or call frequency <80% were filtered.
In order to mapping SNPs to the physical position of genome, a local BLAST program (Altschul et al., 1990 (link)) was performed. The sequences of retained SNPs were queried against the B. napus genome sequences (Chalhoub et al., 2014 (link)). The top and unique blast-hits were selected for the further analysis.
In order to mapping SNPs to the physical position of genome, a local BLAST program (Altschul et al., 1990 (link)) was performed. The sequences of retained SNPs were queried against the B. napus genome sequences (Chalhoub et al., 2014 (link)). The top and unique blast-hits were selected for the further analysis.
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