A plasmid bearing the p53 R248W mutation (pCMV-Neo-Bam p53 R248W) was purchased from Addgene (Cambridge, MA, USA). The open reading frame was cloned into the pEF vector97 (link) along with a FLAG epitope. The pEF-FLAG-MBP plasmid was previously described43 (link). The following oligonucleotide pairs were annealed and ligated into BsmB1-cut pFgh1tUTG98 (link).
ATM exon 2: 5′-TCCCTTGTTTCAGGATCTCGAATC-3′, 5′-AAACGATTCGAGATCCTGAAACAA-3′
TP53 exon 1: 5′-TCCCTCGACGCTAGGATCTGACTG-3′, 5′-AAACCAGTCAGATCCTAGCGTCGA-3′
PRKDC exon 1: 5′-TCCCGCCGGTCATCAACTGATCCG-3′, 5′-AAACCGGATCAGTTGATGACCGGC-3′
RAD51A exon 2: 5′-TCCCTAGCTCCTTCTTTGGCGCAT-3′, 5′-AAACATGCGCCAAAGAAGGAGCTA-3′
ATM exon 2: 5′-TCCCTTGTTTCAGGATCTCGAATC-3′, 5′-AAACGATTCGAGATCCTGAAACAA-3′
TP53 exon 1: 5′-TCCCTCGACGCTAGGATCTGACTG-3′, 5′-AAACCAGTCAGATCCTAGCGTCGA-3′
PRKDC exon 1: 5′-TCCCGCCGGTCATCAACTGATCCG-3′, 5′-AAACCGGATCAGTTGATGACCGGC-3′
RAD51A exon 2: 5′-TCCCTAGCTCCTTCTTTGGCGCAT-3′, 5′-AAACATGCGCCAAAGAAGGAGCTA-3′
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