Dried samples were ground to a fine powder for soluble sugar and sucrose analysis. The sample powder (~0.2g) with three replications was extracted using 6 mL of 80% (v/v) ethanol for 30 min in a water bath at 80°C, then the supernatant was collected after centrifugation at 5,000g for 10 min. This extraction procedure was repeated three times. The three supernatants were pooled and then diluted with 80% ethanol to 25 mL for the measurement of soluble sugar and sucrose content. Soluble sugar content was determined by using the anthrone reagent method and calculated based on the absorbance at a wavelength of 625 nm and a standard curve [39 (link)]. Sucrose content was measured by using the resorcinol method and estimated on the basis of the absorbance at a wavelength of 480 nm and a standard curve [40 (link)]. The mobilized soluble sugar content was calculated by the difference between the largest sugar content and the sugar content at 30 days after anthesis.
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