Generation and Characterization of Genetically Modified Mice

BALB/c and C57BL/6 mice (female, 6–8 weeks old) were purchased from Jackson Laboratories (Bar Harbor, Maine, USA). A TFEB^flox mouse line was generated using the ES cells from EUCOMM depository in Europe, which are the same cells used for generation of liver-specific TFEB knockout mice.24 (link) LysM-Cre mice were purchased from Jackson Laboratories and crossed with TFEB^flox mice to generate MΦ-specific TFEB knockout (MΦ-TFEB^-/-) mice. MΦ-specific TFEB transgenic (MΦ-TFEBtg) mice were generated and characterized previously.25 (link) Female FVB/N mice were bred with male heterozygous C3(1)/SV40Tag mice (a gift from Dr Jeffrey Green, National Cancer Institute). All mice were housed in the University of South Carolina or Washington University in St. Louis Animal Research Facilities.

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Li Y., Hodge J., Liu Q., Wang J., Wang Y., Evans T.D., Altomare D., Yao Y., Murphy E.A., Razani B, & Fan D. (2020). TFEB is a master regulator of tumor-associated macrophages in breast cancer. Journal for Immunotherapy of Cancer, 8(1), e000543.

Publication 2020

BALB/c C57BL/6 mice LysM-Cre mice

C57bl mice Cells Female Heterozygous Knockout mice Liver Male Mice Tfeb Transgenic

Corresponding Organization : University of South Carolina

Other organizations : Washington University in St. Louis, Nanjing Drum Tower Hospital

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Variable analysis

independent variables

Genotype (BALB/c, C57BL/6, TFEB^flox, LysM-Cre, MΦ-TFEB^-/-, MΦ-TFEB^tg, FVB/N, C3(1)/SV40Tag)
Sex (female)
Age (6-8 weeks old)

dependent variables

Not explicitly mentioned

control variables

Animal housing (University of South Carolina or Washington University in St. Louis Animal Research Facilities)

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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