For control A, human dermal fibroblasts (HDFs) from the facial dermis of a 36-year-old Caucasian female (Cell Applications Inc.) were used to establish iPSCs (201B7; Passage 20–29, YA9; Passage 15–24). The 201B7 iPSCs were kindly provided by Dr. Yamanaka
[15 (link)]. A skin-punch biopsy from a healthy 16-year-old Japanese female obtained after written informed consent (Keio University School of Medicine) was used to generate the control B iPSCs (WD39; Passage 8–17). PA iPSCs (PA1, 9, and 22; Passage 10–19) and PB iPSCs (PB1, 2, 18, and 20; Passage 8–17) were generated from a 71-year-old Japanese female patient and a 50-year-old Japanese male patient, respectively, using the same methods used to generate control B iPSCs. The maintenance of HDFs, lentiviral production, retroviral production, infection, stem cell culture and characterization, and teratoma formation were performed as described previously
[14 (link),15 (link)]. All of the experimental procedures for skin biopsy and iPS production were approved by the Keio University School of Medicine Ethics committee (Approval Number: 20-16-18) and Juntendo University School of Medicine Ethics committee (Approval Number: 2012068). hESCs (KhES-1; Passage 29–38 (kindly provided by Dr. Norio Nakatsuji) were cultured on feeder cells in iPS culture media
[43 (link)].
[15 (link)]. A skin-punch biopsy from a healthy 16-year-old Japanese female obtained after written informed consent (Keio University School of Medicine) was used to generate the control B iPSCs (WD39; Passage 8–17). PA iPSCs (PA1, 9, and 22; Passage 10–19) and PB iPSCs (PB1, 2, 18, and 20; Passage 8–17) were generated from a 71-year-old Japanese female patient and a 50-year-old Japanese male patient, respectively, using the same methods used to generate control B iPSCs. The maintenance of HDFs, lentiviral production, retroviral production, infection, stem cell culture and characterization, and teratoma formation were performed as described previously
[14 (link),15 (link)]. All of the experimental procedures for skin biopsy and iPS production were approved by the Keio University School of Medicine Ethics committee (Approval Number: 20-16-18) and Juntendo University School of Medicine Ethics committee (Approval Number: 2012068). hESCs (KhES-1; Passage 29–38 (kindly provided by Dr. Norio Nakatsuji) were cultured on feeder cells in iPS culture media
[43 (link)].
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