Optimization of Prenylated Stilbenoids from Peanut Hairy Roots

Hairy root line 3 used in this study was previously established from peanut cv. Hull [9 (link)] and maintained in 250 mL flasks with 50 mL of MSV media as previously described. Nine-day-old peanut hairy roots were used for yield optimization of prenylated stilbenoids in the culture medium. Prior to elicitation, the spent medium was removed and replaced with 50 mL or 100 mL of fresh MSV medium containing 3% sucrose with different concentration of elicitors as described below. All elicitations were carried out at 28 °C under continuous darkness. For the time course experiment, aliquots of medium from multiple time points were collected from the same flask of elicited hairy root culture.