Breast Cancer Cell Transfection Protocol

Cells were grown as previously described in ref. 17 (link). Briefly, MCF-7 and LCC9 cells were grown in ‘hormone-depleted’ medium: phenol-red free IMEM (Gibco, ThermoFisher) supplemented with 5% dextran-coated charcoal-stripped fetal bovine serum (DCC-FBS, Atlanta Biologicals)^{17 (link)} and concomitantly transfected with either anti-miR-29a, pre-miR-29b-1-3p, pre-miR-29a-3p, pre-miR™ negative control #1 or anti-miR™ negative control #1 for a 48 h, as indicated.

Free full text: Click here

Muluhngwi P., Alizadeh-Rad N., Vittitow S.L., Kalbfleisch T.S, & Klinge C.M. (2017). The miR-29 transcriptome in endocrine-sensitive and resistant breast cancer cells. Scientific Reports, 7, 5205.

Publication 2017

DCC-FBS

Anti mir Biologicals Cells Charcoal Dextran Hormone

Corresponding Organization :

Other organizations : University of Louisville

Top 5 similar protocols

Variable analysis

independent variables

Anti-miR-29a
Pre-miR-29b-1-3p
Pre-miR-29a-3p
Pre-miR™ negative control #1
Anti-miR™ negative control #1

dependent variables

Not explicitly mentioned

control variables

MCF-7 and LCC9 cell lines
Hormone-depleted medium: phenol-red free IMEM (Gibco, ThermoFisher) supplemented with 5% dextran-coated charcoal-stripped fetal bovine serum (DCC-FBS, Atlanta Biologicals)
48 h transfection duration

controls

Pre-miR™ negative control #1
Anti-miR™ negative control #1

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!