CCK-8 kits (CK04, Dojindo, Kumamoto, Japan) were utilized for evaluating the proliferative potential of MUM-2B and MUM-2B/CDDP cells. MUM-2B and MUM-2B/CDDP cells in the logarithmic growth phase were seeded in a 96-well plate (1 × 104 cells per well) and cultured for 1, 2, 3, 4, and 5 days. During this period, 10 μL CCK-8 reagent was loaded at the same time every day, and incubation was made at 37 °C for 3 h. The OD value of each well at 450 nm was measured using a microplate reader. The values were proportional to the number of proliferated cells in the medium, and the cell growth curve was plotted.
MUM-2B and MUM-2B/CDDP cells in logarithmic growth phase were seeded in a 96-well plate (1 × 104 cells per well) for 24 h. After 24 h, cells were exposed to different concentrations of CDDP. After 48 h, 10 μL CCK-8 reagent was loaded for another 3 h of incubation at 37 °C. The OD value of each well at 450 nm wavelength was recorded on a Microplate reader and finally presented in the cell growth curve. IC50 refers to the concentration of cisplatin inhibiting the cell viability by 50%. A higher IC50 value indicates higher resistance to chemotherapy [26 (link)].
MUM-2B and MUM-2B/CDDP cells in logarithmic growth phase were seeded in a 96-well plate (1 × 104 cells per well) for 24 h. After 24 h, cells were exposed to different concentrations of CDDP. After 48 h, 10 μL CCK-8 reagent was loaded for another 3 h of incubation at 37 °C. The OD value of each well at 450 nm wavelength was recorded on a Microplate reader and finally presented in the cell growth curve. IC50 refers to the concentration of cisplatin inhibiting the cell viability by 50%. A higher IC50 value indicates higher resistance to chemotherapy [26 (link)].
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