Western Blot Analysis of Key Proteins

Western blot analysis was performed as previously reported 29 (link). The primary antibodies used were against MCP-1 (1:100, ab157808; Abcam), VCAM-1 (1:200, sc-374429; Santa Cruz) and MMP-2 (1:50, ab178681; Abcam). β-Actin (1:200, sc-25778; Santa Cruz) was used as a loading control. Alexa Fluor 480 (1:10,000, ab175772; Abcam) was used as the secondary antibody. Immunoreactive bands were visualized with Odyssey Software (version 1.2; LI-COR, Lincoln, NE, USA). Protein expression was measured using ImageJ analysis software (NIH, Bethesda, MD, USA).

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Li X., Guo S., Xu T., He X., Sun Y., Chen X., Cao S., Si X., Liao W., Liao Y., Han Y, & Bin J. (2020). Therapeutic ultrasound combined with microbubbles improves atherosclerotic plaque stability by selectively destroying the intraplaque neovasculature. Theranostics, 10(6), 2522-2537.

Publication 2020

ab157808 sc-374429 ab178681 sc-25778 ab175772

Actin Antibodies Antibody Mcp 1 Mmp 2 Protein Vcam 1 Western blot analysis

Corresponding Organization :

Other organizations : Nanfang Hospital, Guangzhou Regenerative Medicine and Health Guangdong Laboratory, Southern Medical University, First Affiliated Hospital of Zhengzhou University

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Variable analysis

independent variables

Primary antibodies used against MCP-1, VCAM-1, and MMP-2

dependent variables

Protein expression levels of MCP-1, VCAM-1, and MMP-2

control variables

β-Actin (1:200, sc-25778; Santa Cruz) as a loading control

controls

No positive or negative controls were explicitly mentioned.

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