Methionine Oxidation in Irradiated Samples

Three months after irradiation, bags were filled with a 50 µM solution of methionine in buffer (10 mM NaH₂PO₄, 10 mM Na₂B₄O₇•10H₂O, 5 mM NaN₃, pH 8.2). After storage for 10 days sampling was performed and the solution analyzed with an Agilent 1260 HPLC equipped with a quaternary pump (G1311C), an autosampler (G1329B), and a fluorescence detector (G1321B). Separation between the methionine and its sulfoxide form was carried out on an Agilent Poroshell HPH-C18 column (4.6 mm × 100 mm, 2.7 μm particles) used with a UHPLC guard Poroshell HPH-C18, 4.6 mm pre-column. Details of chemicals and reagents used, HPLC system and conditions are described in a previous article (Girard-Perier et al., 2020a (link)).

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Girard-Perier N., Marque S.R., Dupuy N., Claeys-Bruno M., Gaston F., Dorey S., Fifield L.S., Ni Y., Li D., Fuchs W.K., Murphy M.K., Pillai S.D., Pharr M, & Nichols L. (2022). Effects of X-Rays, Electron Beam, and Gamma Irradiation on Chemical and Physical Properties of EVA Multilayer Films. Frontiers in Chemistry, 10, 888285.

Publication 2022

1260 HPLC Poroshell HPH-C18 column

Buffer Fluorescence Hplc Irradiation Methionine Nan3 Sulfoxide

Corresponding Organization :

Other organizations : Institut de Chimie Radicalaire, Institut Méditerranéen de Biodiversité et d'Ecologie Marine et Continentale, Pacific Northwest National Laboratory, Texas A&M University

Top 5 similar protocols

Variable analysis

independent variables

Irradiation (time after irradiation)

dependent variables

Methionine concentration
Methionine sulfoxide concentration

control variables

50 μM methionine solution
10 mM NaH2PO4
10 mM Na2B4O7•10H2O
5 mM NaN3
PH 8.2
Agilent 1260 HPLC with quaternary pump, autosampler, and fluorescence detector
Agilent Poroshell HPH-C18 column (4.6 mm × 100 mm, 2.7 μm particles) with UHPLC guard Poroshell HPH-C18, 4.6 mm pre-column

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