Elemental quantification was done as follows20 (link). Urine was collected by housing each mouse in metabolic cages (CLEA Japan) after 1 week acclimation. For serum samples, whole blood was collected from the abdominal aorta and incubated for 30 min at room temperature. Serum was then collected by centrifugation. Each element was quantified either by Xylidyl Blue-I (Mg, Wako) or ICP-ES (Mg, Ca, Na, and K; ICPS-8100; Shimadzu).
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