DNA was extracted using a QIAmp DNA Mini kit (QIAGEN, Hilden, Germany) according to the manufacturer’s instructions. DNA was used to construct a library using a Nextera XT DNA Library Prep Kit (Illumina, San Diego, CA) [12 (link)]. Library pools were then loaded on an Illumina NextSeq CN500 sequencer for 75 cycles of single-end sequencing of 20 million reads for each library, and sequence data were compared with those in a current bacterial and fungal database (NCBI; ftp://ftp.ncbi.nlm.nih.gov/genomes).
Gao W., Lin Y., Yue H., Chen W., Liu T., Ye J., Cai Q., Ye F., He L., Xie X., Xiong G., Wu J., Wang B., Wen W, & Lei W. (2022). Bacteriological analysis based on disease severity and clinical characteristics in patients with deep neck space abscess. BMC Infectious Diseases, 22, 280.
Other organizations :
The First Affiliated Hospital, Sun Yat-sen University, Sun Yat-sen University, First People's Hospital of Foshan, Sixth Affiliated Hospital of Sun Yat-sen University, Third Affiliated Hospital of Sun Yat-sen University, Sun Yat-sen Memorial Hospital, Zhongshan People's Hospital, Guangzhou First People's Hospital, Guangzhou Medical University, The First People's Hospital of Zhaoqing, Jiangmen Central Hospital
Library construction using Nextera XT DNA Library Prep Kit
Sequencing on Illumina NextSeq CN500 for 75 cycles of single-end sequencing
dependent variables
Sequence data compared with a current bacterial and fungal database (NCBI)
control variables
Manufacturer's instructions for DNA extraction and library preparation were followed
controls
No positive or negative controls were explicitly mentioned in the provided information.
Annotations
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