RT-PCR for OGG1 Gene Expression

One μg of RNA and reverse transcriptase (Roche Molecular Biochemicals, Indianapolis, IN, United States) were used.
RT-PCR. The SuperScript III One-Step RT-PCR System with Platinum Taq (Invitrogen, Carlsbad, CA, United States) was used. One microgram of total RNA and primers specific to ogg1 gene were used to amplify OGG1 (Darbinian et al., 2020 (link)).

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Darbinian N., Darbinyan A., Merabova N., Kassem M., Tatevosian G., Amini S., Goetzl L, & Selzer M.E. (2023). In utero ethanol exposure induces mitochondrial DNA damage and inhibits mtDNA repair in developing brain. Frontiers in Neuroscience, 17, 1214958.

Publication 2023

reverse transcriptase SuperScript III One-Step RT-PCR System with Platinum Taq

Gene Ogg1 Platinum Primers Reverse transcriptase Rt pcr

Corresponding Organization :

Other organizations : Temple University, Yale University, Prevea Health, Medical College of Wisconsin, Temple College, The University of Texas at Austin

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Variable analysis

independent variables

1 μg of RNA

dependent variables

Expression of ogg1 gene

control variables

Reverse transcriptase (Roche Molecular Biochemicals, Indianapolis, IN, United States)
SuperScript III One-Step RT-PCR System with Platinum Taq (Invitrogen, Carlsbad, CA, United States)

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