Glycoside Detection in R. hirtellus

Ahuja et al.,³⁸ method was used. Briefly, concentrated H₂SO₄ (1 mL) was added to 500 μL of R. hirtellus stock solutions. After vigorous shaking, the mixture was allowed
to stand for 2 min. The presence of glycosides is indicated by the
reddish-brown color’s appearance.

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Ishtiaq H., Ahmad B., Zahid N., Bibi T., Khan I., Azizullah A., Ahmad K., Murshed A., Rehman S.U., Abdel-Maksoud M.A., El-Tayeb M.A., Lu J, & Zaky M.Y. (2024). Phytochemicals, Antioxidant, and Antidiabetic Effects of Ranunculus hirtellus Aerial Parts and Roots: Methanol and Aqueous Extracts. ACS Omega, 9(20), 21805-21821.

Publication 2024

Corresponding Organization : Guangdong Medical College

Other organizations : Abbottabad University of Science and Technology, COMSATS University Islamabad, King Saud University, Beni-Suef University

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Variable analysis

independent variables

Volume of concentrated H2SO4 (1 mL)

dependent variables

Appearance of reddish-brown color (indicating the presence of glycosides)

control variables

Volume of R. hirtellus stock solution (500 μL)
Time allowed to stand (2 min)

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