Cell line growth and differentiation

The following cell lines and media for routine growth of these cell lines were used: C20 (a kind gift from Dr. Alvarez-Carbonell [25 (link)])—DMEM with 4.5 mg/mL glucose, no pyruvate (Gibco, Thermo Fisher Scientific, Waltham, MA, USA), 5% fetal bovine serum (FBS), antibiotics; HMC3 and SK-N-SH (American Type Culture Collection [ATCC])—EMEM with 2 mM L-glutamine, 1 mM sodium pyruvate (ATCC), 10% FBS, and antibiotics; A172 and THP-1 (ATCC)—RPMI-1640 without L-glutamine (Gibco, Thermo Fisher Scientific, Waltham, MA, USA), 10% FBS, 2 mM L-alanyl-L-glutamine dipeptide (GlutaMAX; Gibco, Thermo Fisher Scientific, Waltham, MA, USA), antibiotics; BHK-ABCA1 (a kind gift from Dr. Chongren Tang [26 (link)]) and BHK-ABCA7 [10 (link)]—DMEM with 4.5 mg/mL glucose, no pyruvate (Gibco, Thermo Fisher Scientific, Waltham, MA, USA), 10% FBS, and antibiotics. All cells were grown at 37 °C in 5% CO₂. For differentiation into macrophages, THP-1 cells were treated with 10 ng/mL PMA (P8139; Sigma-Aldrich, St. Louis, MO, USA) for 24 h.