HDAC1/2 Overexpression in Tau Mice

The mouse HDAC1 or HDAC2 coding sequence was placed into exon 1 of the Tau gene. HDAC2 KO was produced in the laboratory of R.A.D. and engineered to contain loxP recombination sites such that Cre-mediated recombination deletes exons 5 and 6. Sodium butyrate (Sigma) was dissolved in saline. HDAC inhibitors were dissolved in DMSO in 50mg/ml and diluted with saline immediate before injection (100μl–150μl, i.p.). Lysates for immunoblotting were prepared as previously described^{4 (link)}. Immunoblot data were quantified by measuring the band intensity using NIH imaging software and UN-SCAN-it gel digitizing software (Silk Scientific). Immunostaining was performed as described previously^{4 (link)} using LSMeta10 software and a confocal microscope (Zeiss). All behavioral testing was performed as described before^{4 (link)}. The data were analyzed by unpaired Student’s t-test. Two-way ANOVA was employed to compare difference between groups in several time points. Error bars present s.e.m.