RNA sequencing data were generated by Szaniawski et al. [19 (link)]. The sequencing data are publicly available at the National Center of Biotechnology Information under accession no. GSE158434.
Briefly, CD14+ monocytes from healthy donors were cultured for 7 days to allow differentiation to MDM. MDM were stimulated in triplicate with 25 ng/mL of a single interferon (IFN-α, IFN-ε, IFN-γ or IFN-λ) or infected with 250 ng HIV-1-BAL-HSA virus. Total RNA was isolated 18 h following interferon stimulation or 6 h post infection using the RNeasy minikit (Qiagen, Hilden, Germany). Intact poly(A) RNA was purified and stranded mRNA sequencing libraries were prepared using the Illumina (San Diego, CA, USA) TruSeq Stranded mRNA Library Preparation kit (catalog No. RS-122-2101 and RS-122-2102). On an Illumina HiSeq 2500 instrument (HCSv2.2.38 and RTA v1.18.61), a 50-cycle single-read sequence run was performed using HiSeq SBS kit v4 sequencing reagents (catalog no. FC-401-4002).
Briefly, CD14+ monocytes from healthy donors were cultured for 7 days to allow differentiation to MDM. MDM were stimulated in triplicate with 25 ng/mL of a single interferon (IFN-α, IFN-ε, IFN-γ or IFN-λ) or infected with 250 ng HIV-1-BAL-HSA virus. Total RNA was isolated 18 h following interferon stimulation or 6 h post infection using the RNeasy minikit (Qiagen, Hilden, Germany). Intact poly(A) RNA was purified and stranded mRNA sequencing libraries were prepared using the Illumina (San Diego, CA, USA) TruSeq Stranded mRNA Library Preparation kit (catalog No. RS-122-2101 and RS-122-2102). On an Illumina HiSeq 2500 instrument (HCSv2.2.38 and RTA v1.18.61), a 50-cycle single-read sequence run was performed using HiSeq SBS kit v4 sequencing reagents (catalog no. FC-401-4002).
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