Isolation and Characterization of Skeletal Muscle Cells

SF extract was obtained from Research Center for Anti-Aging Technology Development (Busan Technopark, Busan, Korea). Extraction method of SF was indicated by previous research.15 (link),21 (link),22 (link) SF was dissolved in dimethyl sulfoxide as a 20 mg/mL stock solution and diluted with medium prior to use. The following antibodies were purchased from the individual providers: mTOR (sc-136269), p-mTOR (#2971), 4E-BP1 (#9452), p-4E-BP1 (#2855), P70S6K (#2708), p-P70S6K (#9234), myosin heavy chain 3 (MYH3, sc-53091), MuRF1 (ab172479), p-FOXO1 (#9461), and GAPDH (MB001) antibodies were purchased from Cell Signaling (Danvers, MA, USA), Santa Cruz Biotechnology Inc. (Dallas, TX, USA), Bioworld (St Louis Park, MN, USA), and Abcam (Cambridge, UK). Secondary antibodies of antimouse (ADI-SAB-300-J) and antirabbit (ADI-SAB-100-J) were bought from Enzo Life Sciences (Farmingdale, NY, USA). Collagenase, dispase II, basic fibroblast growth factor, and DEX were bought from Sigma-Aldrich Co. (St Louis, MO, USA).

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Kim C.H., Shin J.H., Hwang S.J., Choi Y.H., Kim D.S, & Kim C.M. (2016). Schisandrae fructus enhances myogenic differentiation and inhibits atrophy through protein synthesis in human myotubes. International Journal of Nanomedicine, 11, 2407-2415.

Publication 2016

p-4E-BP1 P70S6K p-P70S6K ADI-SAB-300-J ADI-SAB-100-J Collagenase dispase II basic fibroblast growth factor DEX

4e bp1 Antibodies Basic fibroblast growth factor Collagenase Dimethyl sulfoxide Dispase ii Gapdh Mtor Murf1 Myosin heavy chain P70s6k

Corresponding Organization :

Other organizations : Pusan National University, Pusan National University Yangsan Hospital, Dong-Eui University

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Variable analysis

independent variables

SF extract concentration

dependent variables

MTOR phosphorylation
4E-BP1 phosphorylation
P70S6K phosphorylation
Myosin heavy chain 3 (MYH3) expression
MuRF1 expression
FOXO1 phosphorylation

control variables

Dimethyl sulfoxide (DMSO) as a vehicle control
Collagenase
Dispase II
Basic fibroblast growth factor
Dexamethasone (DEX)

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