Rice (Oryza sativa L.) wild-type (WT) cultivar ‘Nipponbare’, Xoo-resistant cultivar ‘Asominori’, RSV-resistant cultivar ‘Sainokagayaki’ and two transgenic plant lines (BSR1-OX-5 and -9) were grown under greenhouse conditions at 27°C to 30°C. BSR1-OX-5 and BSR1-OX-9 correspond to the previously reported AK070024:OX-5 and AK070024:OX-9 (Dubouzet et al. 2011 (link)), respectively.
For disease resistance tests, except for B. glumae, dehusked seeds were surface sterilized, sown on one-half-strength MS medium (Wako Pure Chemicals, Osaka, Japan), containing 3% (w/v) sucrose and 0.4% (w/v) Gelrite (Wako Pure Chemicals), in Agripots and grown in the growth chamber at 28°C in the dark for 3 days, then at 25°C under long-day conditions (16 h light [60–70 μmol m−2 s−1]/8 h dark) for 4–7 days. For transgenic seeds, Hygromycin B (30–50 μg/ml; Wako Pure Chemicals) was added to the medium. WT seedlings and hygromycin-resistant transgenic seedlings were transplanted into soil (Bonsol No. 2, Sumitomo Kagaku Kougyo, Osaka, Japan) and used for disease resistance tests.
For disease resistance tests, except for B. glumae, dehusked seeds were surface sterilized, sown on one-half-strength MS medium (Wako Pure Chemicals, Osaka, Japan), containing 3% (w/v) sucrose and 0.4% (w/v) Gelrite (Wako Pure Chemicals), in Agripots and grown in the growth chamber at 28°C in the dark for 3 days, then at 25°C under long-day conditions (16 h light [60–70 μmol m−2 s−1]/8 h dark) for 4–7 days. For transgenic seeds, Hygromycin B (30–50 μg/ml; Wako Pure Chemicals) was added to the medium. WT seedlings and hygromycin-resistant transgenic seedlings were transplanted into soil (Bonsol No. 2, Sumitomo Kagaku Kougyo, Osaka, Japan) and used for disease resistance tests.
