Sodium phosphate buffer saline (PBS, 0.1 M, pH = 7.4), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), DMEM-F12 medium, senescence cells histochemical staining kit, and trypsin were received from Aldrich (Poznań, Poland). Hydrochloric acid (HCl, 35–38%) and 2-propanol (98%) were received from Chempur (Piekary Śląskie, Poland). Physiological saline without Ca&Mg (PBS, PAA, Poland), Annexin-V apoptosis assay (BioLegend, San Diego, CA, USA), Annexin V Binding Buffer (BD Biosciences, San Jose, CA, USA), and propidium iodide solution (BD Biosciences, San Jose, CA, USA) were used as received. Normal human dermal fibroblasts (NHDF) were obtained from Lonza (Lonza; Celllab, Warsaw, Poland). Malignant melanoma cells (Me45) and a human metastatic melanoma variant of the WM164 cell line (451-Lu) were obtained from the collection of the Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology (Gliwice, Poland). Spontaneously immortalized human epidermal keratinocytes (HaCaT) were purchased from CSL Cell Line Service GmbH (Eppelheim, Germany). Copolymers were synthesized and self-assembled to encapsulate active substance in aqueous solutions [23 (link),24 (link)] or conjugated with FA and LA [25 (link)] according to previously described procedures.
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