The
iM-forming oligonucleotide (C6T;Figure 2 ) used was synthesized
by and purchased from Midland Certified Reagent Co., Inc. (Midland,
TX). C6T is a good model iM because it undergoes a simple two-state
transition from folded to unfolded form.36 (link) C6T iM oligonucleotide stock was stored in a 10 mM Tris, 1 mM ethylenediaminetetraacetic
acid (EDTA) buffer at pH 8.0 in a −20 °C freezer. The
sodium cacodylate, Tris-HCl, and EDTA used to create buffer solutions
were purchased from Fisher Scientific (Pittsburgh, PA). Putrescine,
spermidine, and spermine were purchased from Sigma Aldrich (St. Louis,
MO).
iM-forming oligonucleotide (C6T;
by and purchased from Midland Certified Reagent Co., Inc. (Midland,
TX). C6T is a good model iM because it undergoes a simple two-state
transition from folded to unfolded form.36 (link) C6T iM oligonucleotide stock was stored in a 10 mM Tris, 1 mM ethylenediaminetetraacetic
acid (EDTA) buffer at pH 8.0 in a −20 °C freezer. The
sodium cacodylate, Tris-HCl, and EDTA used to create buffer solutions
were purchased from Fisher Scientific (Pittsburgh, PA). Putrescine,
spermidine, and spermine were purchased from Sigma Aldrich (St. Louis,
MO).
