Comparative Oocyte Transcriptome Analysis

Total RNA was extracted from 25 wild-type or D6Ertd527e1^–/– fully-grown oocytes from 8–10-week-old animals using PicoPure RNA Isolation Kit with on-column genomic DNA digestion according to the manufacturer’s instruction (Qiagen). RNA-Seq libraries were constructed using the Ovation RNA-Seq system V2 (NuGEN) followed by Ovation Ultralow Library system (DR Multiplex System, NuGEN). RNA-Seq libraries were pooled and sequenced using 65-nt single-end-sequencing using Illumina HiSeq. D6Ertd527e1^−/− oocyte sequencing data were deposited in GEO (https://www.ncbi.nlm.nih.gov/geo/) as GSE213820. Cricetulus griseus oocyte sequencing data were also deposited under GSE213820. Remaining RNA-seq data were published previously and were obtained from GEO database: Mus musculus data accession: GSE116771 [27 (link)], Mesocricetus auratus data accession: GSE116771 [12 (link)] and GSE169528 [33 (link)], Rattus norvegicus data accession: GSE137562 [35 (link)], and Acomys cahirinus data accession: PRJNA436818 [36 ].