A cold, 10% neutral buffered formalin solution (Sigma, St. Louis, MO) was used for fixation of the left lungs. Lungs were inflated and immersed in the formalin solution prior to storage. Slides were then prepared by Histo-Scientific Research Laboratories (Mount Jackson, VA) and blindly reviewed and scored using a validated histology scoring system that has been previously described57 (link). Each slide received three scores from 1–4 based on the degree of cellular infiltration and tissue damage in the parenchymal, peribronchial, and perivascular regions.
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Lung Tissue Fixation and Histological Scoring
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Corresponding Organization : Children's Hospital of Pittsburgh
Other organizations : University of Pittsburgh Medical Center, University of Pittsburgh, Tulane University
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Variable analysis
independent variables
- Fixation method (using 10% neutral buffered formalin solution)
- Degree of cellular infiltration and tissue damage in the parenchymal region
- Degree of cellular infiltration and tissue damage in the peribronchial region
- Degree of cellular infiltration and tissue damage in the perivascular region
- Lungs were inflated and immersed in the formalin solution prior to storage
- Slides were prepared by Histo-Scientific Research Laboratories (Mount Jackson, VA)
- Slides were blindly reviewed and scored using a validated histology scoring system
- No positive or negative controls were explicitly mentioned in the input protocol.
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