Maintenance of Embryonal and Testicular Cell Lines

Human Embryonal Carcinoma cell line NTERA-2 and the murine testicular cell lines TM4 (Sertoli), GC-1 (Spermatogonia), and GC-2 (Spermatocytes) were maintained in DMEM (Gibco, Paisley, UK), supplemented with 10% fetal bovine serum (FBS; Gibco BRL, Italia), 1% L- Glutamine and 1% of penicillin – streptomycin (Gibco, Paisley, UK) [25] , [26] (link), [27] (link), [28] (link). Olaparib (AZD2281) and P005091 were provided by SelleckChem. Cycloheximide, cisplatinum, H2O2 (H1009), erastin (E7781), ferrostatin-1 (SML0583) and desferoxamine (D9533) were from Sigma-Aldrich, Inc (St. Louis, CA, USA). Z-VAD-fmk (FMK001) was from MedChemExpress.

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Morra F., Merolla F., Zito Marino F., Catalano R., Franco R., Chieffi P, & Celetti A. (2021). The tumour suppressor CCDC6 is involved in ROS tolerance and neoplastic transformation by evading ferroptosis. Heliyon, 7(11), e08399.

Publication 2021

DMEM L- Glutamine penicillin – streptomycin Olaparib P005091 Cycloheximide cisplatinum ferrostatin-1 (SML0583) desferoxamine

Azd2281 Cell lines Cisplatinum Cycloheximide Embryonal carcinoma cell Erastin Ferrostatin 1 Glutamine H2o2 Human Murine Olaparib Penicillin Spermatocytes Spermatogonia Streptomycin Z vad fmk

Corresponding Organization : Institute for Experimental Endocrinology and Oncology

Other organizations : University of Molise, University of Campania "Luigi Vanvitelli"

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Variable analysis

independent variables

Olaparib (AZD2281)
P005091
Cycloheximide
Cisplatinum
H2O2 (H1009)
Erastin (E7781)
Ferrostatin-1 (SML0583)
Desferoxamine (D9533)
Z-VAD-fmk (FMK001)

dependent variables

Not explicitly mentioned

control variables

DMEM (Gibco, Paisley, UK) supplemented with 10% fetal bovine serum (FBS; Gibco BRL, Italia), 1% L-Glutamine and 1% of penicillin – streptomycin (Gibco, Paisley, UK)
Human Embryonal Carcinoma cell line NTERA-2
Murine testicular cell lines TM4 (Sertoli), GC-1 (Spermatogonia), and GC-2 (Spermatocytes)

controls

Positive controls not explicitly mentioned
Negative controls not explicitly mentioned

Annotations

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