Standardized Breath Sampling Protocol

All breath samples were collected using a predefined protocol and tested within twenty-four hours. The sampling apparatus was composed of a disposable gas nipple and a sampling bag made of polyether-ether-ketone (PEEK). In this study, we set standard sampling demands and protocols to minimize the influence of the daily diet. Firstly, we conducted sampling at a second visit if he/she was an inpatient and informed the participants to prepare for sampling in advance: no smoking, alcohol, or diets within an hour before sampling. Secondly, participants were required to rinse their mouths with purified water instantly before sampling to minimize the influence of diet, smoking, etc. Thirdly, all samples are required to be collected in the same environment, which could minimize the effects of environmental facts. With a deep nasal inhalation, participants completely exhaled the air into the sampling bag with over 1.2 L volume.

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Fu L., Wang L., Wang H., Yang M., Yang Q., Lin Y., Guan S., Deng Y., Liu L., Li Q., He M., Zhang P., Chen H, & Deng G. (2023). A cross-sectional study: a breathomics based pulmonary tuberculosis detection method. BMC Infectious Diseases, 23, 148.

Publication 2023

Alcohol Diet Inhalation Inpatient Nasal Nipple Polyether ether ketone

Corresponding Organization :

Other organizations : Southern University of Science and Technology, Peking University First Hospital, Peking University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Breath samples

control variables

Time of sample collection (within 24 hours)
Sampling apparatus (disposable gas nipple and PEEK sampling bag)
Participant preparation (no smoking, alcohol, or diets within an hour before sampling, mouth rinsing with purified water before sampling)
Sampling environment (same environment for all samples)

controls

Positive control: None mentioned
Negative control: None mentioned

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