Establishment of Duodenal Organoid Culture

L-WRN cells were obtained from the ATCC (CRL-3276). HEK293-derived Adherent-293 (AD-293) cells were obtained from Stratagene (240085). CBRLuc-mCherry reporter murine duodenal organoids were a gift from Dr. Helen Piwnica-Worms (28 (link)). Murine duodenal organoids were cultured at 37°C in 5% CO₂ and 5% O₂, while all other cell lines were cultured at 37°C in standard 5% CO₂ incubators. All cell lines were authenticated by short tandem repeat profiling and were confirmed to be Mycoplasma free. Recombinant human/mouse Wnt5a was purchased from R&D Biosystems (645-WN-010-CF).

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García García C.J., Acevedo Diaz A.C., Kumari N., Govindaraju S., de la Cruz Bonilla M., San Lucas F.A., Nguyen N.D., Jiménez Sacarello I., Piwnica-Worms H., Maitra A, & Taniguchi C.M. (2021). HIF2 Regulates Intestinal Wnt5a Expression. Frontiers in Oncology, 11, 769385.

Publication 2021

Recombinant human/mouse Wnt5a

Cell lines Duodenal Hek293 cells Human L cells Mouse Mycoplasma Organoids Short tandem repeat Wnt5a Worms

Corresponding Organization : The University of Texas MD Anderson Cancer Center

Other organizations : The University of Texas Health Science Center at Houston, University of Puerto Rico at Río Piedras, University of Puerto Rico at Bayamón

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Variable analysis

independent variables

Wnt5a

dependent variables

Not explicitly mentioned

control variables

Temperature (37°C)
Atmospheric conditions (5% CO2, 5% O2 for murine duodenal organoids, 5% CO2 for other cell lines)
Mycoplasma status (confirmed to be Mycoplasma free)

controls

Positive control: Not specified
Negative control: Not specified

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