Quantifying Intracellular Glycogen and PHB

Commercial analytical kits were used for the quantitation of intracellular glycogen (Total Starch Assay Kit; Megazyme, United States) and intracellular poly-β-hydroxybutyrate (PHB; D-3-Hydroxybutyric Acid Assay Kit; Megazyme, United States) according to the manufacturers’ instructions and Thiel et al. (2017) (link). To avoid the use of Chl a for normalization, the values were calculated as g per cell, by quantitating glycogen and PHB per ml culture, divided by the number of cells per ml culture.

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Vuorio E., Thiel K., Fitzpatrick D., Huokko T., Kämäräinen J., Dandapani H., Aro E.M, & Kallio P. (2021). Hydrocarbon Desaturation in Cyanobacterial Thylakoid Membranes Is Linked With Acclimation to Suboptimal Growth Temperatures. Frontiers in Microbiology, 12, 781864.

Publication 2021

Total Starch Assay Kit D-3-Hydroxybutyric Acid Assay Kit

3 hydroxybutyric acid Assay Cell Glycogen Hydroxybutyrate Intracellular Poly Starch

Corresponding Organization : University of Turku

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Variable analysis

independent variables

Not explicitly mentioned

dependent variables

Intracellular glycogen
Intracellular poly-β-hydroxybutyrate (PHB)

control variables

Not explicitly mentioned

controls

No positive or negative controls were specified by the authors.

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