Direct ELISA using the recombinantly expressed S fragment comprising amino acids 318–510 (S318–510) was performed with the mAbs as described [
22 (link)]. Competition ELISA was performed as follows. Anti-Myc-captured S318–510 fragment of the Frankfurt 1 strain was incubated with nonsaturating amounts of biotinylated IgG in the presence or absence of competing IgG. Bound biotinylated IgG was detected with streptavidin-conjugated-HRP (BD Pharmingen, San Diego, California, United States). Alternatively, S318–510 was captured by CR3014 or CR3022, detected using a biotinylated CR3014 and CR3022, and developed as described above.
22 (link)]. Competition ELISA was performed as follows. Anti-Myc-captured S318–510 fragment of the Frankfurt 1 strain was incubated with nonsaturating amounts of biotinylated IgG in the presence or absence of competing IgG. Bound biotinylated IgG was detected with streptavidin-conjugated-HRP (BD Pharmingen, San Diego, California, United States). Alternatively, S318–510 was captured by CR3014 or CR3022, detected using a biotinylated CR3014 and CR3022, and developed as described above.
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