Incorporation of [14C] oleate into cholesteryl [14C] oleate in cultured cells after oxysterol addition was determined using previously described methods (Goldstein et al., 1983 (link)).
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Protocol detail
Cholesteryl Oleate Synthesis Assay
Corresponding Organization :
Other organizations : The University of Texas Southwestern Medical Center
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Variable analysis
independent variables
- Oxysterol addition
- Incorporation of [14C] oleate into cholesteryl [14C] oleate in cultured cells
- Cultured cells
- Previously described methods (Goldstein et al., 1983)
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