RNA-Seq Analysis of Differential Gene Expression

Expression of each gene was calculated using the RNA-Seq by Expectation-Maximization (RSEM, http://deweylab.github.io/RSEM/, accessed on 13 October 2022). The criteria for DEGs were the same as described in our precious report [59 (link)]. The DEGs were used for gene set enrichment analysis (GSEA) against the Kyoto Encyclopedia of Genes and Genomes (KEGG) database (http://www.genome.jp/kegg/, accessed on 13 October 2022). All the tests were conducted in triplicates. The data were analyzed using SPSS statistical analysis software version 17.0 (SPSS Inc., Chicago, IL, USA).

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Liu Y., Tang Y., Ren S, & Chen L. (2023). Antibacterial Components and Modes of the Methanol-Phase Extract from Commelina communis Linn. Plants, 12(4), 890.

Publication 2023

SPSS statistical analysis software version 17.0

Expression gene Gene Genome Rna seq

Corresponding Organization : Shanghai Ocean University

Other organizations : Virginia Commonwealth University, Hunter Holmes McGuire VA Medical Center

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Variable analysis

independent variables

Not explicitly mentioned

dependent variables

Gene expression levels calculated using RSEM

control variables

All the tests were conducted in triplicates

controls

No positive or negative controls were explicitly mentioned

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