Multiplex Cytokine Profiling of Subjects

We performed a multiplex cytokine bead assay blindly and in parallel using the Bio-plex Pro Human Cytokine assay (Bio-Rad, Hercules, CA) and Milliplex MAP Human Cytokine/Chemokine panel 1 (Millipore, Billerica, MA) according to the manufacturers’ instructions as described previously.^{[15 (link)]} The subjects’ baseline demographic characteristics were compared using Fisher's exact test for discrete variables and the Wilcoxon test for continuous variables. The Kruskal–Wallis test followed by Dunn's multiple comparisons test was used to compare cytokine levels between groups. To rank the cytokine levels, we performed a multivariate classification algorithm termed random forest analysis (RFA),^{[16 ]} using the R package RandomForest (http://cran.r-project.org/web/packages/randomForest/) ver. 4.6–12 software, as previously described.^{[17 (link)]}

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Migita K., Horai Y., Kozuru H., Koga T., Abiru S., Yamasaki K., Komori A., Fujita Y., Asano T., Sato S., Suzuki E., Matsuoka N., Kobayashi H., Watanabe H., Naganuma A., Naeshiro N., Yoshizawa K., Ohta H., Sakai H., Shimada M., Nishimura H., Tomizawa M., Ario K., Yamashita H., Kamitsukasa H., Kohno H., Nakamura M., Furukawa H., Takahashi A., Kawakami A., Ohira H, & Yastuhashi H. (2018). Serum cytokine profiles and Mac-2 binding protein glycosylation isomer (M2BPGi) level in patients with autoimmune hepatitis. Medicine, 97(50), e13450.

Publication 2018

Bio-plex Pro Human Cytokine assay Milliplex MAP Human Cytokine/Chemokine panel 1

Assay Chemokine Cytokine Human

Corresponding Organization :

Other organizations : Nagasaki Medical Center, Fukushima Medical University, AbbVie (United States), Pfizer (United States), Nagasaki University, National Hospital Organization Takasaki Medical Center, Higashihiroshima Medical Center, National Hospital Organization, Kanazawa Medical Center, Beppu Medical Center, Nagoya Medical Center, Asahikawa Medical University, Ureshino Medical Center, Okayama Medical Center, Tokyo National Hospital, Kure Medical Center, University of Tsukuba

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Cytokine levels

control variables

None explicitly mentioned

controls

Positive controls: None mentioned
Negative controls: None mentioned

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