Transcriptional Regulation of BCCIP Gene

BCCIP promoter region (926 bp, −724 ~+202 bp; 391 bp, −330 ~+61 bp; 272 bp, −277 ~ −5 bp) was introduced into pGL4-Luc vector. Similar to previously described (Wu et al. 2013 (link)), 293T cells grown on 12-well plates were co-transfected with 0.4 μg of pGL4 which encodes firefly luciferase, 0.12 ng of the control plasmid Renilla luciferase vector which encodes renilla luciferase and effector plasmid expressing pGL4-BCCIP using PEI reagent (Polysciences, U.S.A.). Total effector plasmids in each transfection were adjusted to 0.8 μg with empty vector. 48 h after transfection, the transactivation activity of pGL4-BCCIP was determined by measuring firefly and Renilla luciferase activities using the Dual-Luciferase Reporter assay kit (Promega, U.S.A.).