Detailed descriptions of the materials and methods are provided in SI Appendix and include the following: eukaryotic cell lines; virus supernatants from the HEK293 2089 cell line; collection and quantitation of virus supernatant from the B95-8 cell line; preparation of B cells from adenoid tissue; purification of B cells on MACS columns; primary B cells infection with EBV; TMRE and Annexin V detection; glucose uptake using 2-NBDG; FACS sorting for naïve B cells; analysis of the cells’ diameter; cell cycle analysis; calculation of the cell DI; Western blot protein quantification with the Wes platform technology; FACS staining for surface B cell markers; preparation of cell lysates; sample collection for time-course RNA-seq experiments; RNA isolation; library preparation and sequencing; and bioinformatic analysis, including transcript quantification by Salmon, quality control and data normalization, identification of DE genes, gene clustering, GO analysis, heatmaps of log10-transformed expression data, PCA of phenotypic data, PCA with viral genes, clustering of viral genes, and data availability.