hiPSCs were gifted by Professor Zhiguo Chen (Cell Therapy Center, Beijing Institute of Geriatrics, Xuanwu Hospital Capital Medical University) (18 (link)) and were maintained in mTeSR1 medium (Stemcell Technologies, Inc.; cat. no. 85850) in 6-well cell culture plates (Costar; Corning, Inc.; cat. no. 3516) coated with 1% vol/vol human embryonic stem cell-qualified Matrigel (Corning, Inc.; cat. no. 354277) in a 37˚C incubator at 5% CO2. hiPSCs were passaged in Dissociation Solution for human embryonic stem cells/iPSCs (ReproCELL, Inc.; cat. no. RCHETP002) at a 1:3 ratio every 7 days, according to the manufacturer's protocol.
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Maintaining Human Induced Pluripotent Stem Cells
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Variable analysis
independent variables
- Passage of hiPSCs in Dissociation Solution for human embryonic stem cells/iPSCs
- Maintenance of hiPSCs in mTeSR1 medium
- Culture of hiPSCs in 6-well cell culture plates coated with 1% vol/vol human embryonic stem cell-qualified Matrigel
- Incubation of hiPSCs in a 37˚C incubator at 5% CO2
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