DPI-ELISA was carried out according to the method described by Brand et al. [19 (link)]. The biotinylated MeAGPS1a promoter probe was obtained by PCR, with Biotin-MeAGPS1a primer pairs and KU50 genomic DNA as template (forward: 5′-Biotin-CAGCTGCCCCTACCGTTAA-3′ and reverse: 5′-Biotin-TAGCAAGTTCAGATTTGGAAAAAACC-3′). The ELISA micro-well plates used Pierce® Streptavidin High Capacity Coated Plates (Thermo Fisher Scientific, Rockford, IL, USA). Tag Anti-ProS2 (TaKaRa, Tokyo, Japan) and Goat Anti-Mouse IgG/HRP (Boster, Wuhan, China) were used as the primary and secondary antibodies in the DPI-ELISA.
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