Fourier transform infrared spectroscopy (FTIR) analysis of the silk coatings was performed with a Jasco FT/IR-6200 spectrometer (Easton, MD), equipped with a multiple reflection horizontal Miracle attenuated total reflectance (ATR) attachment (ZnSe crystal, from Pike Tech., Madison, WI). For each measurement, 64 scans were performed each with nominal resolution of 4 cm−1. The secondary structures of the protein samples were identified from the absorption spectra, peak positions of the amide I region (1595–1705 cm−1) were obtained after Fourier self-deconvolution. Curve fitting was performed using Jasco Spectra-Manager Analysis Software (Jasco, Easton MD), as previously described 18 (link).
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FTIR Analysis of Silk Coatings
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Corresponding Organization : Tufts University
Other organizations : Klinikum rechts der Isar, Technical University of Munich
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Variable analysis
independent variables
- Fourier transform infrared spectroscopy (FTIR) analysis
- Absorption spectra
- Peak positions of the amide I region (1595–1705 cm^-1)
- Jasco FT/IR-6200 spectrometer (Easton, MD), equipped with a multiple reflection horizontal Miracle attenuated total reflectance (ATR) attachment (ZnSe crystal, from Pike Tech., Madison, WI)
- 64 scans with nominal resolution of 4 cm^-1
- No positive or negative controls were explicitly mentioned.
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