MicroPET/CT scan was performed on a SIEMENS Inveon scanner (Siemens, Germany). U87MG tumor bearing mice (n = 5) were intravenously injected with 3.7 MBq (200 μCi) of 18F-tLyP-1. MicroPET/CT images were acquired as 10-min static images at 30, 60, and 120 min after the injection with the mice under isoflurane anesthesia. The images were reconstructed by a 3-dimensional ordered subsets expectation maximum (OSEM) algorithm and CT correction was applied for attenuation correction.
In the PET images, ROIs were measured with the Inveon Research Workplace (IRW) 3.0 software (Siemens, Germany). The ROI was determined by manually superimposing the ellipsoid volume of interest (VOI) to the target tissue. The activity concentrations were determined by the mean pixel intensity within each VOI, and converted to μCi/mL using a calibration constant. Assuming the tissue density of 1 g/mL, the ROI activity was converted to μCi/g and normalized as percent injected dose per gram (%ID/g). The tumor/normal brain ratios were calculated by dividing the ROI activity in tumor by that in the normal brain [40 (link)].
In the PET images, ROIs were measured with the Inveon Research Workplace (IRW) 3.0 software (Siemens, Germany). The ROI was determined by manually superimposing the ellipsoid volume of interest (VOI) to the target tissue. The activity concentrations were determined by the mean pixel intensity within each VOI, and converted to μCi/mL using a calibration constant. Assuming the tissue density of 1 g/mL, the ROI activity was converted to μCi/g and normalized as percent injected dose per gram (%ID/g). The tumor/normal brain ratios were calculated by dividing the ROI activity in tumor by that in the normal brain [40 (link)].
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