MBIC Modulates Cell Signaling Proteins

Cells were treated with MBIC in a dose-dependent manner for 24 h. Cells were harvested, lysed and applied for Western blot analysis as described previously [10 (link)]. The primary antibodies that were used in this study included: anti-p53, anti-survivin, anti-phospho-survivin (anti-p-survivin), anti-Cdk1, anti-cyclin B1, anti-Bax, anti-caspase-3, anti-caspase-7, anti-caspase-9 (1:1000) (Cell Signaling Technology, USA) and mouse anti-β-actin (1:40000) (Sigma-Aldrich, St. Louis, MO, USA) antibodies. Western blot images were quantified and processed by ImageJ software (NIH, USA). Each experiment was done in triplicate.

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Hasanpourghadi M., Pandurangan A.K., Karthikeyan C., Trivedi P, & Mustafa M.R. (2017). Mechanisms of the anti-tumor activity of Methyl 2-(-5-fluoro-2-hydroxyphenyl)-1 H-benzo[d]imidazole-5-carboxylate against breast cancer in vitro and in vivo. Oncotarget, 8(17), 28840-28853.

Publication 2017

anti-p53 anti-survivin anti-Cdk1 anti-cyclin B1 anti-Bax anti-caspase-3 anti-caspase-7 anti-caspase-9 mouse anti-β-actin

Actin Anti b1 Antibodies Caspase 3 Caspase 7 Caspase 9 Cdk1 Cells Cyclin Cyclin b1 Mbic Mouse Survivin Western blot Western blot analysis

Corresponding Organization :

Other organizations : University of Malaya, Rajiv Gandhi Technical University

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Variable analysis

independent variables

MBIC dose

dependent variables

P53 expression
Survivin expression
Phospho-survivin (p-survivin) expression
Cdk1 expression
Cyclin B1 expression
Bax expression
Caspase-3 expression
Caspase-7 expression
Caspase-9 expression

control variables

Cell culture conditions (e.g., incubation time, temperature)
Lysis and Western blot procedures

controls

Positive control: β-actin (1:40000)

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