Cyclophosphamide-Induced Diabetes in NOD Mice

Cyclophosphamide has been shown to rapidly promote a high incidence of diabetes in NOD mice.15 (link),16 (link) At 11 weeks of age, 70 mice had blood drawn for baseline A_1c, and then received 200 mg/kg of Cyclophosphamide (Sigma-Aldrich, St Louis, MO, USA) by intraperitoneal injection. All mice were screened for blood glucose levels three times weekly; by 13 weeks of age, 25 mice with two consecutive blood glucose levels >300 mg/dL were diagnosed as diabetic and entered into the study, and the remaining mice received a second 200 mg/kg intraperitoneal dose of Cyclophosphamide. By 15 weeks of age, an additional 14 mice (39 total) had two consecutive blood glucose levels >300 mg/dL, were diagnosed as diabetic, and were entered into the study.