The longissimus dorsi and semimembranosus muscles were cut to measure the meat quality traits according to previous reports (3 (link), 16 (link)). Briefly, the pH values were measured at 24 h (pH24h) after slaughter. Meat color measurements cover indicators L* (lightness), a* (redness), and b* (yellowness) after slaughter 24 h (L24h, a24h, and b24h). Drip loss, cooking loss, crude protein, and ash content were determined as described previously (3 (link), 17 (link)). Amino acid profile and fatty acid composition were determined using gas chromatography–mass spectrometry (GC–MS 7890B-5977A, Agilent, Palo Alto, CA, United States) and liquid chromatography–mass spectrometry (Liquid phase was performed on Thermo Ultimate 3,000 system, Thermo Fisher Scientific Inc., Waltham, MA, United States; Mass Spectrometry was performed on Thermo Q Exactive Focus mass spectrometer, Thermo Fisher Scientific Inc., Waltham, MA, United States), respectively.
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